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Image Search Results
Journal: bioRxiv
Article Title: VPS34-IN1 inhibits cap-mediated translation and synergizes with STING to drive type-I IFN expression in human plasmacytoid DCs
doi: 10.1101/2024.06.17.599308
Figure Lengend Snippet: ( A ) LC3B lipidation was analyzed by determining the LC3-II/LC3-I ratio for CAL-1 cells treated with VPS34-IN1 for 8 h and bafilomycin for 4 h. LC3-I, nonlipidated form; LC3-II, lipidated form. ( B ) CAL-1 cells treated with VPS34-IN1 were stained with MitoSOX and analyzed by flow cytometry. The mean fluorescence intensity was compared to untreated cells (ctr). ( C ) Immunofluorescence confocal microscopy of VPS34-IN1-treated CAL-1 cells showing EEA1 (red) and LAMP1 (blue) distribution (n = 3). Scale bar, 5 μm or 1 μm in magnified areas. ( D ) Cell viability was determined for cells treated with varying concentration of VPS34-IN1 by propidium iodide exclusion and flow cytometry. ( E - I ) Cells were stimulated with CL307 and VPS34-IN1 or spautin-1. ( E, G, H ) Levels of IFNB1 and TNF mRNA were normalized to housekeeping gene ( GAPDH ) and fold change determined in comparison with untreated cells (ctr). ( F ) Immunoblot for phosphorylated STAT1 (Tyr 701 ). Levels of pSTAT1 relative to β-actin were compared against untreated cells (ctr). ( I ) Secretion of IFN-β and TNF-α was monitored by ELISA. ( A , B , D - I ) Bars show the mean ± SD and each dot shows data from one experiment. ns, non-significant results, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by one-way ANOVA with Šidák post hoc test (A), paired Student’s t test (B), one-way ANOVA with Dunnett post hoc test (D) and one-way ANOVA with Tukey post hoc test (E-I).
Article Snippet: Human TNF-α was quantified in cell supernatants using a commercially available ELISA kit from PeproTech (900-TM25) and the appropriate commercial buffers (
Techniques: Staining, Flow Cytometry, Fluorescence, Immunofluorescence, Confocal Microscopy, Concentration Assay, Comparison, Western Blot, Enzyme-linked Immunosorbent Assay
Journal: bioRxiv
Article Title: A chemically defined and xeno-free hydrogel system for regenerative medicine
doi: 10.1101/2024.05.28.596179
Figure Lengend Snippet: (A) ELISA of culture medium for Apolipoprotein B (APOB), taken from iHeps 2 days after the end of the hepatic differentiation protocol, and from PHH culture media 2 days after plating. (B) Brightfield microscopy of 3D tubular branching strictures observed when Laminin 411 concentrations are increased. Scale bar = 100μm. (C) Alkaline Phsophatase (ALP) activity measured by colourimetric assay on culture medium taken 2 days the end of the hepatic differentiation protocol.
Article Snippet: APOB secretion in the supernatant was quantified using the
Techniques: Enzyme-linked Immunosorbent Assay, Microscopy, Activity Assay